Highlights:Technical interest is aimed application of genetic tools for development in tissue culturing, phenotypical, microbiological and molecular characterization of pathogens.

Skills:Hand on Basic molecular tools, Good use of Biotechnology tools, PCR, Virology and molecular Diagnostics, Plant Pathology

Goal:To see to all round upliftment of the organization, work and serve with all diligence, bringing to bear the knowledge, training and skills acquired.

Laboratory Technician 04/2017 - 07/2023
International Institute of Tropical Agriculture, Ibadan, Oyo Nigeria
Industry: Agricultural research
I am passionate on providing solutions to plant pathogens affecting plants.
? Contribute to microbial analysis in carrying over efficacy trial of Aflasafe SNOI in Senegal; o Isolation of Aspergillus strains from soil, maize and groundnut. o Water vial and silica saves of isolates o Inoculation of isolates on different medium o Identification Aspergillus strains with polyphasic approach (morphology & microscopy) o Generation and purification of mutants o Evaluation of isolates with different Vegetative compatibility group(VCG) • (Complementation) o Analysing the percentage recovery of the isolates from the fungi o Preparation of different type/form of growth media (general purpose & selective medium) ? Assist in carrying out molecular characterization in Aspergillus strains from Zimbabwe, Congo, Niger, Sudan, Zimbabwe using polymerase chain reaction (PCR); o Single sporing of isolates o Extraction of high quality DNA from Aspergillus strains (culture) o Nucleic acid quality and quantity assessment using agarose gel electrophoresis and Nanodrop spectrophotometer respectively o CAPs multiplex-PCR assay to monitor indels in the aflatoxin biosynthesis gene cluster o Resolution of amplified PCR products on agarose gel o o Taking Gel image with Gel doc. System o o Monitoring deletions patterns ? Molecular characterization of Fusarium spp. o Isolation of Fusarium o Morphological identification of Fusarium spp o DNA isolation from Fusarium cultures o Use of molecular tool in detecting fumonisin-producing specie of Fusarium ? Assist in computation and proper management of collected data; ? Assist in maintaining the functionality of the laboratory; ? Perform any other duties as may be assigned by the supervisor.--
Research Technician 10/2015 - 03/2017
International Institute of Tropical Agriculture, Ibadan, Oyo Nigeria
Industry: Agricultural research
I have gained experience in Virology and molecular diagnostics, protein expression and cloning
? Assist in maintaining tissue culture of Africa yam clone for virus cleaning in the laboratory; ? Assist in maintaining mother plants in the screen house; ? Assist in propagating yam plants from tissue culture to screen-house for hardening ? Phenotyping of yam disease(YMV, YMMV, BADNA & Anthracnose) to enhance yam breeding to increase productivity and improved quality in West Africa o Visiting several yam fields for phenotypical scoring of virus and fungi symptoms o Developing assay to correlate field scores to laboratory diagnostics o Extraction of high quality DNA from plants tissues and fungi cultures o Virus indexing of yam diseases using polymerase chain reaction (nPCR, RT-PCR, qPCR) o Resolution of amplified PCR products on agarose gel o Quantitative assessment of viral titre values of the samples collected from field with ACP-ELISA, PAS-ELISA, DAS-ELISA and TAS-ELISA o Computation and proper management of collected data o Proper dissemination of needed information from available data ? Assist in pathogenicity test for Colletotrichum gleosporoides on yam mapping population o Isolation of Colletotrichum gleosporoides from infected leaf o Organism establishment on growth medium o Harvesting fungal spores for inoculation o Quantification of spore suspension with Hemacytometer o Inoculation of inoculum (spore suspension) on plants in screen house and on detached leaf (in-vitro) o Proper monitoring of humidifier with proper computation of the humidity and temperature in the humidifier o Good quality images of plants at different stage of the experiment and proper documentation o o Analysis of Detached-leaf assay with leaf-doctor ? Perform any other responsibilities assigned by the supervisor--

The Polytechnic Ibadan 09/2012 - 09/2014
Ibadan, Oyo, Nigeria
Degree: Associate's Degree
Major:Science Laboratory Technology
I graduated with Upper credit

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