Highlights:

Skills:Biochemist, Protein Biologist, Biophysics, Molecular Biology

Associate Research Scientist 04/2020 - current
Yale University, West Haven, CT United States
Protein biochemist in the lab of Dr. James Rothman working on biochemical models of neuronal exocytosis.
• Mentored projects for junior postdoctoral associates. • Used single vesicle assays to determine fundamental role of diacylglycerol lipid in C1 domain-containing chaperone, Munc13. • Developed methodology using vesiculated membranes from live cells to validate “cell on a chip” device. Key Accomplishments: Listings in 2 upcoming publications and 1 patent. Mentored 2 junior postdoctoral associates.--
Postdoctoral Associate 01/2016 - 03/2020
Yale University, West Haven, CT United States
Protein biochemist developing models to study neuronal exocytosis in the lab of Dr. James Rothman.
• Developed and troubleshooted protocols to produce neuronal SNARE chaperone proteins harnessing bacterial and mammalian technologies. • Designed and developed protein constructs (including non-natural amino acids) capable of single-site labeling for anisotropy and FRET studies. • Performed quantitative binding studies with Microscale Thermophoresis (MST) to determine recruitment of SNAP25 by Munc13. • Solved a long-standing problem of expressing the SNARE protein Syntaxin1a complexed with its chaperone Munc18. Key Accomplishments: Mentored 2 graduate students, developed protocols to express a novel protein complex resulting in new projects, grant submissions and listings in 3 publications.--

Drexel University 09/2003 - 07/2008
Philadelphia, PA, United States
Degree: Bachelor's Degree
Major:Biomedical Engineering
BSc in Biomedical Engineering

Login to view resume: Resume_Kalyana Sundaram -