Highlights:

Skills:Real Time PCR, Nucleic Acid extraction and amplification, Assay validation & verification, CAP, ISO15189, Polymerase Chain Reaction

Laboratory Specialist 01/2013 - current
Prince Sultan Military Medical City, Riyadh, Saudi Arabia
Industry: Hospital
Sharp-eyed, Motivated, highly skilled and reliable molecular biology technologist with over 13 years of experience in Molecular Laboratory field. Highly organized and diligent with a systematic approach to handling specimens, analysing, and reporting reliable and accurate results. Proven ability to develop and execute complex protocols, troubleshoot technical issues, and maintain laboratory equipment. Seeking a position in an organization where I can utilize my skills and contribute to cutting-edge technologies.
• Execute moderate to high complexity testing in Virology and Molecular Infectious disease according to established policy for all phases of testing: pre-analytical, analytical, and post-analytics. • Perform high complexity testing, results, and QA for all specimens for Molecular Testing. • Demonstrated the ability to communicate test results effectively with physicians, pathologists, and nursing staff. • Consistently commended for the timely, and accurate completion of Molecular Assay of the patient specimen. • Ensured compliance with hospital policies and laboratory procedures, including maintaining the cleanliness of laboratory equipment, instruments, and work area. • Ensured compliance with CAP, CBAHI, JCI, and CLIA requirements by adhering to strict quality control guidelines and standard operating procedures within the laboratory. • Have a sophisticated knowledge of clinical instrumentation and standard protocols for clinical laboratory analysis, CAP CLIA. • Trained laboratory technologists and oversaw the appropriate implementation of diagnostic testing for infectious diseases, and annual competency training. • Conduct Qualitative Detection of SARS-CoV-2 and MERS-CoV from the respiratory specimen. • Carry out Quantitative Analysis of Infectious disease (HIV, HCV, HBV, and HCV Genotype) by using Real-Time PCR (Abbott Molecular Diagnostics). •--
Supevisor-Molecular Biology 01/2008 - 01/2013
Quest Diagnostics, Gurgaon, India
Industry: Healthcare
Molecular Diagnostics
• Developed qualitative detection of Mycobacterium Tuberculosis and Ankylosing Spondylitis (HLAb27) using conventional PCR followed by Gel Electrophoresis. • Working Knowledge of microarray, NGS, and Sanger Sequencing method • Performed manual extraction of Nucleic Acid (DNA/RNA) from blood samples, urine, body fluids, Bacterial cells & tissue samples using QIAamp kit. • Performed Quantitative detection of BCR/ABL, PML RARA bcr1, and PML RARA bcr3, CBFb/MYH11 inv (16) using IPSOGEN amplification kit on ABI 7500 Fast Real-Time PCR System. • Performed Quantitative detection of BCR-ABL using qPCR BCR-ABL1 Quant kit from Asuragen on ABI 7500 Fast Real-Time PCR. • Performed Extraction of DNA using BioRobot EZ1 Advance using EZ1 DNA Blood Kit from QIAGEN. • Performed Detection of HPV High Risk & HPV 16/18 genotyping by CERVISTA Kit from third wave technologies. • Represented Molecular Biology Department in Successful Accreditations of CAP (College of American Pathology), ISO-15189 & NABL (National Accreditation Board for Testing and Calibration Laboratories) as Laboratory Supervisor. • Developed standard training program & trained new staff for handling various instruments and different molecular assays. • Periodically evaluates methodology, makes recommended changes, and assists in test interpretation--

Magadh University 05/2000 - 12/2003
, , India
Degree: Bachelor's Degree
Major:Chemistry
Chemistry

CV MOHAMMAD ALAM Molecular