Highlights:

Skills:RNA extraction and cDNA preparation, DNA amplification and purification, PCR, Quantitative real-time PCR, Cell culture, RNA interference (RNAi), Bioassays for LC50 determination, Size-exclusion chromatography, R Program, High-throughput data analysis, Transmission & Scanning Electron Microscopy, Biodiversity indices calculations, Fluorescence microscopy, Microsoft Office, GraphPad Prizm, SPSS, Unix operating system

Postdoctoral Researcher 08/2022 - 07/2023
University of Texas at Austin, Austin, Texas United States
Industry: Academia and Research
I had my postdoc in Marcotte's Lab at the University of Texas at Austin. I Worked with Dictyostelium discoideum to identify and characterize novel proteins and protein complexes. We identified the Commander Protein complex and a novel actin-binding protein.
(i) Identified a previously uncharacterized protein in Dictyostelium discoideum (Dicty). (ii) Cross-linked Dicty proteins and identified the Commander Protein complex. (iii) Prepped proteins for size-exclusion chromatography & LCMS to study protein interactions. (iv) Cultured and prepped Dicty and Escherichia coli for proteomic analysis. (v) Mentored undergraduate and graduate students. (vi) Helped draft manuscript/review article for publication.--
Doctoral Researcher 09/2018 - 06/2022
Hebei Normal University, Shijiazhuang, Hebei China
Industry: Academia and Research
I worked in Hebei Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology, College of Life Sciences, Hebei Normal University. I worked with ticks and my research direction was on molecular and physiological ecology. I discovered bioactive molecules in Cymbopogon citratus essential oil that can be used as a natural acaricide. I unraveled the molecular mechanism of their actions in ticks after determining the LC50 for each tick life stage via bioassays. I also identified influential phosphoprotein in tick saliva that facilitates blood-feeding and pathogen transmission.
(i) Identified plant bioactive molecules with potential for tick control. (ii) Analyzed gene expression profiles and performed loss-of-function studies (RNAi) on ticks. (iii) Discovered a mechanism of action (Using Omics and RNAi) which revealed disruption of Ca2+ homeostasis in ticks leading to mitochondrial depolarization. (iv) Identified phosphoproteomes in tick saliva that contribute to successful hematophagy and pathogen transmission. (v) Sampled ticks in Xiaowutai Mountains, prepped & sequenced them; identified tick endo-symbionts with detoxification capacity, & studied epigenetics linked to cold adaptation. (vi) Published more than 10 first-author articles including one review.--

Hebei Normal University 09/2018 - 06/2022
Shijiazhuang, Hebei, China
Degree: Doctoral Degree
Major:Molecular & Physiological Ecology
Minor:Vector Biology, Ecology & Control
I studied and conducted my doctoral research at Hebei Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology, Hebei Collaborative Innovation Center for Eco-Environment, Ministry of Education Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang, Hebei 050024, PR China

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