Highlights:Work Experience RELIANCE INDUSTRIES LIMITED Navi Mumbai 1. Research Scientist May 2018 till present 2. Research Assistant Dec 2014 to Apr 2018 EMBIO RESEARCH CENTRE Mahad, Raigad 1. Research Assistant Mar 2010 to Nov 2012 2. Research Officer Dec 2012 to Nov 2014

Skills:Microbial fermentation,, Upstream bioprocess, Bioanalytical, Enzymology

Goal:With more than 14+ years of experience in research and development within industrial projects. I am a highly-motivated individual, with a strong background in microbiology (aerobic and anaerobic), bacteria and yeast fermentation with hands-on experience in molecular biology, enzymology, and metabolite analysis (GC, GCMS, and HPLC) interested in working in an area that provide enough opportunities to learn and contribute knowledge that results in high impact and quality. I am an effective thinker and fast learner, possessing excellent communication and interpersonal relationship skills, and can easily cope with a new atmosphere

Certification:MIT Institute of Design, Pune, India (Jun 2022 - June 20223) Advanced Certificate In Innovation & Experience Design Google UX Design Certificate (May 2022 - Sep 2022)

Honor:Secured third place at the Oral Presentation Competition during the National Conference on Recent Advances in Biotechnology organized by Manipal Institute of Technology in Manipal, India, on October 31, 2023.

Research Officer 03/2010 - 11/2015
Embio Limited, Raigarh, Maharashtra India
Industry: Biotechnology
Fermentation & Biotransformation Work Enzymes and extracellular metabolite studies:
Fermentation & Biotransformation Work • The process was developed for high bacterial biomass production (Shake flask to fermentor), which was used for expressing Transaminase and assay methods for quantitative and qualitative analysis of the enzyme. • Isolation and partial purification of Aceto Hydroxy Acid Synthase enzyme from bacteria for the production of chiral drug intermediates. • Yeast-based production of phenyl acetyl carbinol (PAC) production using biotransformation of benzaldehyde and acetaldehyde in the bioreactor. Enzymes and extracellular metabolite studies: • Isolation of crude enzymes from bacteria and yeast cells, and developing assays for enzymes like Pyruvate Decarboxylase, Invertase, Alcohol dehydrogenase, Aceto Hydroxy Acid Synthase (AHAS), and Transaminase. • Enzyme kinetics: Determination of Km and Vmax values of Pyruvate decarboxylase for pyruvate & benzaldehyde substrates.--
Research scientist 12/1015 - current
Reliance Industries Limited, Mumbai, Maharashtra India
Industry: Biotechnology
With more than 14+ years of experience in research and development within industrial projects. I am a highly-motivated individual, with a strong background in microbiology (aerobic and anaerobic), bacteria and yeast fermentation with hands-on experience in molecular biology, enzymology, and metabolite analysis (GC, GCMS, and HPLC) interested in working in an area that provide enough opportunities to learn and contribute knowledge that results in high impact and quality.
Reliance Industries Limited 1.Hyaluronic acid (HA) production from bacterial fermentation (Streptococcus zooepidemicus) •Developed strains to produce low molecular weight Hyaluronic Acid using various mutagenic procedures (UV, NTG, and EMS). •Optimized nutritional and physical parameters for the synthesis of low molecular weight HA. •Implemented different strategies, such as H2O2 acclimatization, at the fermentor level (3.0L) to enhance low molecular weight HA production. 2.Lactic acid (HA) production from bacterial fermentation (Lactobacillus rhamnosus) •Cultivated and propagated Lactobacillus cultures from lab inventory and new cultures obtained from culture collection centers (shake flask study). • Optimized fermentation processes for lactic acid production in fermenters ranging from 1 to 10 liters (achieved 80% carbon efficiency). 3. Biological coal bed methane (Bio-CBM) •Generated methane biogenically from coal using synthetic consortia and consortium isolated from various environments at the bottle level. •Conducted coal adaptation studies and large-scale experiments (45L Bio-reactor) to study carbon balance. •Scaled up Bio-CBM production in 45 L bio-reactor and Core Flood Reactor (CFR) using coal-specific consortia. 4.Microbial culture maintenance •Standardized protocols for lyophilization of bacterial and yeast cultures using freeze dryer. •Managed maintenance of existing and newly added cultures in the lab inventory. 5.Additional Responsibilities •Analyzed samples (GC, GC-MS, HPLC) and compiled data for reports. • Presented findings in monthly review meetings. •Authored a blog on the Learnet platform titled "Biohydrogen Production: Strategies to Improve Process Efficiency through Microbial Routes." •Developed and recorded a comprehensive Web-based training (WBT) ILT course titled "Microbiology of Anaerobic Microorganisms" on the R-University platform.--

CSIR-National Chemical Laboratory 01/1018 - 03/2024
PUNE, Maharashtra, India
Degree: Doctoral Degree
Major:Biotechnology
Research Title: Strain improvement for enhanced erythritol production by Moniliella pollinis using cost-effective substrate.

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