Highlights:1. Biomedical Research Scientist with 15+ years of pre & post-Ph.D. experience in Cancer Biology, Molecular Biology, Molecular Medicine, Pre-clinical molecular toxicology, Human molecular cytogenetics, and genomics. 2. Expertise in dissecting molecular signaling mechanisms of cancer responses to targeted therapy, and signaling crosstalk in acquired resistance to therapeutics using various 2D, 3D culture-based, and animal models. 3. I am currently investigating Kras-driven lung adenocarcinoma progression and drug resistance mechanism in 3D-lung organoid models by targeting mutant Kras in mouse alveolar epithelial type 2 (AT2) cell-derived lung organoids by combinatorial drug screening and CRISPR validation strategies. 4. Mentoring and teaching experience for Ph.D., master's, and undergraduate students. 5. Multiple project management experience at diverse national and international institutes with multicultural environments, well-developed innovation, collaboration, and problem-solving skills with tenacious work ethics. 6. Ability to work independently and as a part of multidisciplinary teams with strong organizational, written, and oral communication skills.

Skills:Project management, creative problem solving,, cloning, Viral vector designing, CRISPR prime editing, Animal models, Preclinical toxicology assays, Basic Flowcytometry, protein-protein interactions, Co-Immunoprecipitation, ELISA, PCR/RT-PCR/Q-PCR, Immunofluorescence staining, Immunohistochemistry, Light and Confocal Microscopy, preparation of parameters for preclinical studies, Teaching/Mentoring, Scientific Publications, Public speaking, Excellent oral/written skills, PC and Mac operating systems, Microsoft office, data collection from public databases and analysis, Endnote, Prism-Graphpad, Snapgene, Cross-functional collaborations, Tenacious work ethics,, Organized data, Clean records, Lab archives, Different modes of drug Injections (IP, SC, IT, IV, Western blotting, Lentiviral and Retroviral packaging, transfection optimization and validation, 3D cultures methods design, 3D-Cancer stem cell cultures, generation of lung organoids from mouse and human, Mammalian cell cultures, Isolation of primary cells from human and animal t, Cell line maintenance, cell immortalization, cryopreservation, Site-directed mutagenesis, Gene expression analysis, CRISPR knockouts, development of tumor biomarkers, cytotoxicity assays, Molecular toxicology assays, Molecular target discovery

Goal:My career goal as a Cancer biology research scientist is to leverage my pragmatic approach and extensive experience in cancer research to contribute significantly to advancements in the understanding and treatment of this complex disease. With over 15 years of pre-and post-Ph.D. research experience, I aim to further enhance my expertise in project management, leading interdisciplinary teams towards impactful discoveries. I am dedicated to maintaining a continuous commitment to learning and staying abreast of emerging developments in the field, ensuring that my work remains at the forefront of scientific innovation. My ultimate objective is to play a pivotal role in shaping the future of cancer research, driving progress, and improving outcomes for patients through my dedication, knowledge, and collaborative spirit.

Membership:1. Global Leadership Panel Member Fight Cancer Global. 2. Alumni of Taiwan International Graduation Program. 3. American Association for Cancer Research (Associate member ID: 368269). 4. Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB) member. 5. Member of the Cancer Epigenetic Society.

Certification:1. Flow cytometry data analysis and visualization. 2. nSolver 101: basic analysis from Nanostring Inc. 3. SciPhD certification program; The business of science for scientists.

Honor:1. Best Poster Presentation Award in Laboratory of Cancer Biology and Genetics (LCBG) Scientific Retreat 2023, held at National Institutes of Health, Bethesda, MD from 16-17 November 2023. 2. NIH Visiting Fellowship Award for Post-Doctoral Research-NCI/NIH, Bethesda, MD, USA (2019-2024). 3. Outstanding Poster presentation award in Institutional Research Day poster competition 2017, Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan. 4. Young Scientist Travel Award by the Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB) in association with the Taiwanese Society for Biochemistry and Molecular Biology (TSBMB) to participate in the Young Scientist Program (November 24-26, 2015) and the 14th FAOBMB Congress (November 27-30, 2015) in Hyderabad, India. 5. Taiwan International Graduate Program (TIGP) funding for Ph.D. Studies at Academia Sinica, from Government of Taiwan, 2012. 6. Prof. Áskell Löve award for best research paper presentation in “XI All India Conference on Cytology and Genetics” held at Bangalore University from 23-25 January 2012. 7. Best Poster Presentation Award in “XI All India Conference on Cytology and Genetics” held at Bangalore University from 23-25 January 2012. 8. Summer Research Fellowship by the Indian Academy of Sciences in 2008. 9. Gold Medal for best academic performance in Biology, Akkineni Nageswara Rao College, Gudivada, Andhra Pradesh, India 2001.

Visiting Postdoctoral Research Fellow 11/2019 - current
National Cancer Institute (NCI), NIH, Bethesda, MD United States
Industry: Federal Government
• Establishment of Murine AT2-cell derived 3D-Lung Organoid Models to Investigate the Mutant Kras-driven Lung Adenocarcinoma Progression and Drug Resistance Mechanisms. • Developing CRISPR-Cas9 Edited AT2 Organoid Models for Modelling Lung Adenocarcinoma with Multigenetic Lesions.
Handled and executed creative multiple research projects. ? Designed, constructed, and optimized candidate gene expression vectors in vitro and in vivo using a variety of cell-based, Organoid, and animal models. ? Fostered cross-functional collaborations and coordinated with technical and non-technical support teams for a smooth flow of Research projects to finish projects promptly. ? Established Lung cancer organoids, and cancer stem cell 3D culture systems to screen anticancer drugs in vitro and in vivo. ? Maintained up-to-date knowledge of emerging technologies and scientific approaches. ? Designed 2D and 3D cancer cell culture systems to investigate the role of KRAS target proteins and dissect their signaling pathways using specific drug inhibitors. ? Optimized CRISPR Prime gene-editing technology to generate point mutations in KRAS oncogene in lung cancer cell lines and validated mutation frequencies by Insilco quantitative assessment tools. ? Optimized CRISPR-mediated multiple gene targeting in Lung stem cells grown in a 3D culture system. ? Prepared summaries of data and presented them internally to colleagues and group leaders. ? Drafted SOPs, and protocols, diligently documented experimental data in electronic files, and authorized scientific reports for internal documentation or regulatory submissions. ? Contributed to high-impact research publications, reviewing manuscripts, making scientific posters and presentations at scientific conferences and meetings; actively supported a culture of innovation, learning, respect, and trust.--
Postdoctoral Research Fellow 02/2019 - 09/2019
Institute of Biomedical Sciences Academia Sinica, Taipei, Taiwan, Taipei, Taiwan Taiwan
Industry: Government
• Molecular characterization of co-chaperone functions and cancer stemness of IFIT genes which can predict the clinical outcome of DNA damaging agents in oral squamous cell carcinoma (OSCC).
Elucidating the IFIT gene family molecular signatures can predict the clinical outcome of cancer therapies that are targeted to the OSCC patients who are likely responding to the particular therapeutic drugs. Developing therapeutic markers for OSCC patients. Translating in vitro findings to mouse models of disease by establishing molecular biomarkers. Roles & Responsibilities: ? Designed and Managed the entire research project single-handedly. ? Presented progress of the project and scientific concepts in multiple National and International conferences. ? Coordinated with technical and non-technical support teams to ensure the smooth flow of projects as per committed timelines. ? Mentored Ph.D., Master's, Research Assistants, and Summer Interns students. ? Wrote research publications and reviewed technical reports and project progress reports. Technical Skills: Cloning, Gene silencing by siRNA & shRNA, Study of Gene/Protein expression, Mammalian/Cancer stem cell cultures, SDS-PAGE, Co-Immunoprecipitation, Confocal Microscopy, Flow cytometry, LC-MS/MS data analysis by MASCOT search software, Histopathology, Immunohistochemistry, Immunoblot, Immunofluorescence, Quantification of Protein expression in cancer tissues by Panoramic 250 flash III whole slide scanner and 3DHISTECH Software. In vitro and In vivo Anti-cancer Drug screening, Xenograft, and Orthotopic Mice models for Cance cell invasion, metastasis, and drug efficacy tests. Protein docking, Protein-protein interactions analysis, Gene sequence analysis, Q-RT-PCR, and Data mining from all publicly available cancer databases.--
Research Technical Assistant 07/2008 - 12/2008
National Institute of Nutrition, HYDERABAD, telangana India
Industry: Government
Investigation of preclinical therapeutic drug response to prepare parameters for phase 1 clinical studies.
Prepared parameters for Phase I clinical studies by determining acute, sub-acute, and chronic toxicity of new drugs by the administration to laboratory animals.Research: Investigation of preclinical toxicity of therapeutic drugs to prepare parameters for Phase I clinical studies. ? Prepared parameters for Phase I clinical studies by determining acute, sub-acute, and chronic toxicity of new drugs by the administration to laboratory animals.--
Research Assistant 12/2008 - 08/2012
Center for DNA Fingerprinting and Diagnostics, Hyderabad, India, HYDERABAD, telangana India
Industry: Government
Projects: • Establishment of EBV transformed cell lines from families with rare genetic disorders. • Cloning, Characterization, and analysis of chromosomal rearrangements in human genetic disorders. • Referral center for detection of genetically modified organisms (GMOs) by employing DNA-based markers.
oles & Responsibilities: ? Utilized Cellular, Biochemical, Cytogenetics, and Molecular biology techniques to characterize genetic disorders. ? Established Fluorescent in situ hybridization technique using Bacterial artificial chromosomes as probes to characterize chromosomal abnormalities. ? Established EBV transformed cell lines from families with rare genetic disorders. ? Developed, validate, and employing DNA-based markers for GMO detection of various crops contains specific transgenes. ? Supported the establishment of genotypic characterization techniques like PCR, qPCR, and western blotting to assess Genetically modified seeds. ?Developed strategies to ensure quality in terms of documentation. ? Provided technical support for lab management. ? Coordinated with technical and non-technical teams to ensure smooth flow of projects as per institute guidelines. ? Presented the progress of the project in various scientific meetings. ?Authored research publications, reviewed technical reports and laboratory-specific SOPs. Key Skills: Establishment of Human Lymphoblastoid cell lines, Human chromosomal preparations, G-banding, Karyotyping, Molecular probe preparation from Bacterial artificial chromosomes, Fluorescent in situ hybridization, Mammalian cell cultures, RT-PCR, primer design, PCR-based screening of GMOs, SDS-PAGE, Immunoblotting, Multiplex-PCR. Genotyping, Human DNA, RNA isolations.--
Lecturer 06/2006 - 04/2008
Akkineni Nageswara Rao college,, HYDERABAD, ANDHRA PRADESH India
Industry: Educational
Worked as Head of the Post Graduate Microbiology Department.
? Managed 75 Postgraduate students, 4 teaching staff, and 2 non-teaching staff. ? Delivered lectures on various life science topics inside and outside academia. ? Efficiently conducted academic exams and invigilation duties resulting in a 90% student pass percentage. ? Coordinated and managed other staff members and colleagues. ? Designed and delivered teaching courses. ? Gave Lectures on various topics in Life science. ? Prepared and presented educational progress reports to the college management--

Acharya Nagarjuna univeristy 06/2001 - 04/2004
GUDIVADA, ANDHRA PRADESH, India
Degree: Bachelor's Degree
Major:Microbiology, Biochemistry, Food and Applied Nutrition
Bachelors in Microbiology, Biochemistry, Food and Applied Nutrition. Grade: A

Vijay_2024 3D- Cancer Organoid models, Animal Models, CRISPR, Preclinical Toxicology, Molecular Target Discover,