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Mduduzi Moyo

Manchester Greater Manchester, United Kingdom

Phone: xxx-xxx-xxxx

Email: xxx@xxxx.xxx



  • Looking For: research associate, medical technologist

  • Occupation: Life, Physical, and Social science

  • Degree: Bachelor's Degree

  • Career Level: Qualified

  • Languages: English, Zulu, Ndebele, Shona

Career Information:

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Highlights:

Skills:Report Writing, Analytical Skills, Project Management, Research, Interpersonal skills, Strong Planning, Strong organizational skills, Strong conceptual skills

Goal:Highly motivated Bachelor of Science graduate with high aspirations of a career in a medical or a research laboratory as a biomedical scientist.

Honor:2009 Highest number of tutor recommendations in all National Diploma Applied Science Trafford College 2009 Highest number of tutor recommendations in National Diploma Applied Science Year 2 Trafford College 2008 Highest Record of Achievement of all students in the Science Department Trafford College 2008 Highest Record of Achievement of all students in National Diploma Trafford College


Experiences:

Lab Research Project 09/2013 - 07/2014
University of Salford, Manchester, United Kingdom
A full year lab research project working independently. This project consisted of working with lung tissue from smokers suffering from Chronic obstructive pulmonary disease (COPD). COPD is identified as an inflammatory disorder characterized by airway and lung inflammation. In this project the lung tissue was analyzed and the results revealed an increase of blood markers such as Interleukin 6. This was expected as Interleukin 6 is a pro-inflammatory cytokine.
The main aim of this project was to use Immunohistochemistry (IHC) to detect the expression of specific markers in COPD patients. These markers are expressed in the inflammatory cells i.e. macrophages. The markers of interest in this project were Cluster of Differentiation 68 (CD68) and Glucocorticoid receptor (GR). GR is a steroid receptor that is expressed in almost every cell in the body and regulates genes controlling development, metabolism, and immune response.CD68 is a glycoprotein that is expressed in macrophages or monocytes. The expression of these markers is different in individuals and can be greatly affected by extrinsic factors for an example cigarette smoke. Cigarette smoke causes irreversible damage and affects physiology and function of many cells in the body. Lung tissue samples from different patient groups underwent IHC and the results were analyzed. The lung tissue samples were provided by North West Lung Centre Wythenshawe Hospital. The samples came from 13 patients, categorized as; current smokers with COPD, ex-smokers with COPD and health non-smokers. To prevent autolysis or putrefaction, the samples were fixated. The lung tissue samples were fixated using the Formalin Fixed Paraffin Embedded Tissues (FFPE) procedure. FFPE preserves the tissue from degradation and maintains the structure of the cells within the tissue. The IHC protocol was completed in two consecutive days and the fixated tissue was stained for CD68 and GR. Three slides were stained for each patient sample [control, CD68 and GR). To completely remove paraffin the slides were then deparaffinized and rehydrated in xylene and ethanol. Failure to completely remove paraffin causes poor staining. After staining all the tissue samples, they were analyzed microscopically using a Leica ICC50 microscope. Images of three field views per patient sample were captured at X400 magnification and cell counts were completed. A transparent containing 100 squares was used for cell counting. Equal numbers of squares were used on every sample for counting. After the cell count, total cell count was calculated by adding positive and negative cells together and the percentage of cells expressing CD68 and GR was calculated.--

Education:

University of Salford 09/2011 - 07/2014
Manchester, , United Kingdom
Degree: Bachelor's Degree
Major:Biomedical Science with Honours
This degree course covered broad biological principles relating to the normal function of the human body, as well as providing up to date training in medical and disease related topics including Pathophysiology, Cell Pathology, Clinical Immunology, Haematology, Microbiology, Genetics and more. This course has a strong practical focus, allowing students to develop the lab skills needed to become an employable biomedical scientist, or for a career in medical laboratory research in a wide range of industry sectors.


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